The peparation 500 or "bouse de corne" occupies an important place in biodynamic agriculture because it pertains to the soil and the roots of the plants. It is obtained by fermenation in the soil during the winter of cow dung introduced into cow horns by particular procedures.  The corns are pulled out of the soil and the dung must be humid, thin, supple, and without odor in order to be used. It is conserved in a ceramic recipient sheltered from the sun, in a trunk of wood, for example.  This is preparation is used at least twice a year, in the spring and fall, a bt before the biological activity in the soil becomes more active. The preparation is pulverised in the evening after being touched up.
This preparation favors microb activity in the soil, the formation of humus and ameliorates the absorption and the retention of water in the soil which regulates the pH (increases it in acidic soils and lowers it in basic soils). It stimulates the germination of grains, the growth of root systems, and helps the dissolution of minerals in the soils.

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